What does TaqMan probe do?
TaqMan® Gene Expression Assays are based on 5′ nuclease chemistry, which uses a fluorogenic probe to enable the detection of a specific PCR product as it accumulates during PCR.
How much does a TaqMan probe cost?
TaqMan – Molecular Beacons – Fluorescent Molecular Probes
| Fluorescent Probes Price List Summary* and PAGE Purified Yield** | ||
|---|---|---|
| 50 nmol scale | 1 umol scale | |
| 5′-Hex, Tet, Cy 3 or Cy 5/Quencher | $230.00 | $450.00 |
| Cy3.5, Cy5.5/Quencher | $390.00 | $650.00 |
| Cy7-NHS, 5′-NHS Dyes (Rox, AF Dyes, other)/Quencher | $410.00 | $750.00 |
How does a 5 ‘- nuclease assay TaqMan assay work?
The 5′-nuclease (TaqMan) assay is a robust single nucleotide polymorphism genotyping method where an allele-specific probe that binds to a perfectly complementary target is cleaved by the 5′-nuclease activity of Taq DNA polymerase.
What is MGB TaqMan?
Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5′ fluorescent reporter dye and a 3′ nonfluorescent quencher (NFQ).
How much do primers cost PCR?
Service Details
| Service Name | Unit | Unit Price |
|---|---|---|
| Primer Synthesis (25 nmol) | 1 Base | $0.53* |
| Primer Synthesis (100 nmol) | 1 Base | $1.05* |
| Primer Synthesis (250 nmol) | 1 Base | $1.88* |
| sgRNA PCR Primer Pair Design & Synthesis Service (25 nmol/primer) | 1 Pair | $148.50 |
What is FAM in real-time PCR?
Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5′ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3′ end.
What is the 5 nuclease assay?
The fluorogenic 5′ nuclease assay is a convenient, self-contained amplification process. The assay uses a fluorogenic probe consisting of an oligonucleotide to which a fluorogenic reporter dye and a non-fluorescent Black Hole Quencher Dye are attached.
Is MGB a quencher?
Custom Applied Biosystems™ TaqMan® MGB (minor groove binder) probes incorporate a 5′ reporter and a 3′ nonfluorescent quencher (NFQ).
What is the advantage of using an MGB probe?
Probes containing a MGB molecule form an extremely stable duplex which gives the probe a significant increase of the melting temperature (Tm). It is therefore possible to use shorter probes (down to 13 bases) in real-time PCR compared to conventional dual labelled probes.
How does TaqMan SNP genotyping work?
The TaqMan® SNP genotyping technology utilizes the 5′ nuclease activity of Taq polymerase to generate a fluorescent signal during PCR. For each SNP, the assay uses two TaqMan® probes that differ in sequence only at the SNP site, with one probe complementary to the wild-type allele and the other to the variant allele.