Does Alcian blue stain cartilage?

Does Alcian blue stain cartilage?

Alcian blue and Alizarin red stain cartilage and bone, respectively. As a cationic dye, Alcian blue binds strongly to sulfated GAGs and glycoproteins, while Alizarin red, an anionic dye, binds to cationic metals such as calcium [1].

What does Alcian blue stain test for?

Alcian Blue is a stain that is used to visualize acidic epithelial and connective tissue mucins. Mucins are a type of carbohydrate and are found in the GI tract and respiratory tract. Acidic mucins have a negative charge.

Is Alcian blue acidic?

Thus the standard Alcian blue – pH 2.5 stains all acidic mucins (Figure 3). In contrast, mucins that contain predominately carboxylated carbohydrates will stain strongly with Alcian blue – pH 2.5 but not with Alcian blue at a pH of 1.0.

What is Alcian blue technique?

PRINCIPLE: Alcian blue is a group of polyvalent basic dyes that are water soluble. The blue color is due to the presence of copper in the molecule. 5), Alcian blue stains both sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

Does Alcian Blue staining hyaluronic acid?

Results: Weakly acidic sulfated mucosubstances, hyaluronic acid, and sialomucins are stained dark blue.

Does Alcian blue stain goblet cells?

However, Alcian blue stains an acidic mucus, which does not stain with PAS-haematoxylin. This suggests that the staining properties of some of the goblet cells have changed. The extra goblet cells revealed by Alcian Blue staining makes the total of goblet cells present close to normal in numbers.

Is Alcian blue a basophilic stain?

… on histochemical staining and cell surface expression we characterized the cultured cells as basophil-like. These cells stain with Alcian blue, a characteristic only seen from human peripheral blood basophils and tissue MCs (Fig 1).

Does Alcian blue staining hyaluronic acid?

What Colour does Alcian blue stain?

Alcian Blue is a basic dye containing copper, which gives it the blue color. The stain molecules carry a positive charge and are attracted to the negative mucins….1) Alcian Blue.

Tissue type Goblet cells
Composition Acid mucins – Simple
Alcian blue 2.5 Positive
Alcian blue 1.0 Negative

Does alcian blue stain goblet cells?

How do you use alcian blue stain?


  1. Bring sections to distilled water.
  2. Stain in the Alcian blue solution 15 mins.
  3. Wash well in running tap water 5 mins (For lower pH solutions ie pH 1.0/0.2, drain and blot dry, to prevent removal of stain in water).
  4. Rinse in distilled water.
  5. Counterstain with neutral red stain 1 min.

Does Alcian blue stain nucleus?

Results: Weakly acidic sulfated mucosubstances, hyaluronic acid, and sialomucins are stained dark blue. Nuclei and cytoplasm are red to pink or pale pink, respectively, if nuclear fast red counterstaining is used.

What is the pH of Alcian blue?

Alcian blue, pH 2.5. Alcian blue solution, pH 2.5: Dissolve 1 g of Alcian blue (8GX) in 100 ml of 3% acetic acid solution. Procedures: 1. Deparaffinize and hydrate to distilled water. 2. Place in 3% acetic acid solution for 5 min followed by staining in Alcian blue solution, pH 2.5 for 30 min. 3.

What is in the Alcian blue staining kit?

The Alcian Blue Staining Kit contains 1% Alcian Blue Solution, 0.1% Nuclear Fast Red in convenient, ready-to-use solutions. At pH 2.5, Alcian Blue stains acid mucopolysaccharides and shows a blue color, while Nuclear Fast Red stains nuclei pink to red and the cytoplasm pale pink [1].

Can Alcian blue and Alizarin Red be used to identify cartilage and bone?

This protocol covers staining of the pre- and postnatal mouse skeleton using Alcian blue and Alizarin red to identify cartilage and bone, respectively. Keywords: Alcian blue, Alizarin red, Cartilage, Bone, Cleared skeletal preparation, Whole-mount

How is Alcian blue extracted from fixed cultures?

Fixed cultures are stained overnight with 0.5% Alcian blue in 0.1 N HCI and rinsed twice with 0.1 N HCI to remove any unbound dye. A final rinse with distilled water is aspirated to near dryness. Alcian blue is then extracted by 4 M guanidine HCI overnight at 4°C. Absorbance values are read at 600 nm after temperature equilibration.