What are the essential requirements for BSL-3?

What are the essential requirements for BSL-3?

However, the common essential features of BSL-3 laboratory include unidirectional air flow using room pressure gradients of negative pressure, exhaust air being HEPA (high efficiency particulate air) filtered and proper procedures for disposal of biomedical waste.

What are BSL-3 practices?

BSL-3 Special Practices

• Entering Work Areas.
• Labels & Signs.
• Restricted Access.
• Medical Clearance.
• Training.
• Containment Devices.
• Centrifuge.
• Sterilization.

What is a Level 3 laboratory?

Biosafety Level 3 (BSL3) laboratories involve research using agents that are associated with serious or lethal human disease for which preventative or therapeutic treatments may be available.

What is required of laboratory personnel in a Biosafety Level 3 laboratory?

Common requirements in a BSL-3 laboratory include: Standard personal protective equipment must be worn, and respirators might be required. Solid-front wraparound gowns, scrub suits or coveralls are often required. All work with microbes must be performed within an appropriate BSC.

How do I set up a BSL-3 lab?

BSL-3 laboratory should be ideally located in separate building or it must be separated from the general traffic flow and accessed through an anteroom/air lock facility within a building. Hence, for construction of BSL-3 laboratory a new stand-alone facility of the institutional site should be selected.

How are the autoclave guidelines different between ABSL 2 and?

How are the autoclave guidelines different between ABSL-2 and -3? An autoclave should be located where infectious materials are used in the ABSL-3 facility, but this is not necessary for ABSL-2. Which statement is TRUE about the prevention or containment of infectious aerosols at ABSL-2 or -3?

What is the difference between bsl2 and bsl3?

Biosafety Level 2 (BSL-2) is suitable for work involving agents that pose moderate hazards to personnel and the environment. A BSL-3 lab is designed to contain an inhalation exposure risk for personnel working in the lab as well as individuals outside the lab.

What is the difference between BSL-3 and 4?

BSL-4 builds upon the containment requirements of BSL-3 and is the highest level of biological safety. The microbes in a BSL-4 lab are dangerous and exotic, posing a high risk of aerosol-transmitted infections. Infections caused by these microbes are frequently fatal and without treatment or vaccines.

What are BSL-3 organisms list?

Organisms. The pathogens that require BSL-3 laboratories include HIV, H1N1 flu, Yersinia pestis, Mycobacterium tuberculosis, SARS, Rabies Virus, West Nile Virus, Ricketts, etc.

What activities are never allowed in lab?

Eating, drinking, smoking, gum chewing, applying cosmetics, and taking medicine in laboratories where hazardous materials are used should be strictly prohibited.

When can I work in BSC?

Working in the BSC: Ensure the vertical sliding sash at the correct height. Allow only one BSCs operator at a time. Sit at the BSC with armpits level with the bottom of the sash. Perform all operations at least 4 inches from the front grille on the work surface.