What is a disposable pipette used for?
What is a disposable pipette used for?
Transfer pipets are disposable plastic pipets used to transfer small volumes of liquids. They consist of a long, slender tube and a molded bulb which can be gently compressed and relaxed to remove or dispense a desired liquid.
What is a serological pipette?
The serological pipette is a nearly ubiquitous laboratory instrument used for transferring milliliter volumes of liquid. Serological pipettes typically have gradations along their sides for measuring the amount of liquid being aspirated or dispensed.
How do you autoclave pipettes?
Autoclaving is the simplest sterilization method if all pipette parts tolerate extreme heat. Pipettes should be autoclaved according to the manufacturer’s instructions. To achieve sterility, a holding time of at least 20 minutes at 121°C (252°F) is required.
What is a single volume pipette?
The single volume or transfer pipet is the most accurate and simplest to use type, but is, obviously, limited to measurement of a fixed, single volume. Generally, these pipets deliver their inscribed volume by complete drainage of the pipet from an etched mark.
Why are pipettes important?
A pipette is the small piece of lab equipment that makes other lab procedures possible. Without a pipette, the transfer of liquids from one location to another would be messy and unsafe. Additionally, there would be no way to track the volume of liquid being used accurately in each pipette tip.
When should you use a serological pipette?
The best time to use serological pipette is when transferring liquids between receptacles, mixing chemical solutions, or laying reagents that are composed of different densities. Each of these procedures requires a high attention to detail when it comes to the aspirating and dispensing of your solution.
What is the difference between a serological pipette and a Mohr pipette?
A Serological pipette is a graduated pipette in which the calibration marks extend all the way to the tip. A Mohr pipette is a graduated pipette in which the calibration marks do not extend to the tip but at a point above the tip.
How do you autoclave tips?
The Best Recipe for Sterilizing Pipette Tips
- Open the lid of the tip tray a tad—propping it up with the lid’s locking tab helps; a little tape can hold it in place—don’t wrap the box in foil!
- Run your dry cycle as normal.
- Let the damp tips dry in the warm autoclave (or transfer to a drying cabinet/incubator)
Why pipette tips should be autoclaved?
Autoclaving is used to sterilize items, such as pipette tips. The process achieves sterilization by removing air which causes steam to become superheated. Sterilization plays a vital role in research and any scientific endeavor using pipette tips.
What is TD and TC?
TC or TD abbreviated for “to contain” and “to deliver” respectively. In a ‘TC’ marked pipette, the contained quantity of the liquid corresponds to the capacity printed on the pipette, While in ‘TD’ marked pipette, the delivered quantity of liquid corresponds to the capacity printed on the pipette.
What is the volume of a pipette?
Typical volumes are 10, 25, and 50 mL. Volumetric pipettes are commonly used to make laboratory solutions from a base stock as well as prepare solutions for titration.