What is K3 EDTA used for?

What is K3 EDTA used for?

K2 EDTA and K3 EDTA are two types of anticoagulants used in routine blood tests. Both consist of potassium ions chelated by EDTA. Furthermore, the main function of both is to prevent blood coagulation during storage. This is by chelating calcium ions in the blood.

Which EDTA salt is recommended for blood counts and morphology?

Historically, ethylene diamine tetraacetic acid (EDTA) has been recommended as the anticoag- ulant of choice for hematological testing, since it allows the best preservation of cellular components and morphology of the blood cells.

How much EDTA do I add to whole blood?

For use as an anticoagulant, disodium or tri-potassium salts of EDTA, the optimal concentration is 1.5 mg per ml of blood.

What are the effects of excessive EDTA?

EDTA can cause abdominal cramps, nausea, vomiting, diarrhea, headache, low blood pressure, skin problems, and fever. It is UNSAFE to use more than 3 grams of EDTA per day, or to take it longer than 5 to 7 days. Too much can cause kidney damage, dangerously low calcium levels, and death.

Which salt form of EDTA is better?

Dipotassium ethylenediaminetetraacetic acid is better than tripotassium salt for electrochemiluminescence insulin measurement.

Does heparin affect CBC?

No negative effects on morphology and staining of blood cells were apparent in smears from heparin samples compared with K2EDTA samples. Within the different values compared, the limits of agreement are small enough to be confident that lithium heparin can be used for routine CBC counts in a clinical setting.

How much blood is in a EDTA tube?

A. Yes, the BD Vacutainer PPT Tube (reference number 362788) contains EDTA anticoagulant and an inert polyester gel. The Hemogard closure color is pearl white and the blood draw volume is 5mL.

How long does blood last in EDTA tube?

It can be stored for 12, 24 or 36 h prior to processing at 4°C and it can be frozen at −80°C for 20 days and then thawed under controlled conditions. Stability of the samples can different based on variety of assays used.

Is K2-or k3-edta better for blood count?

In optimal conditions (appropriate anticoagulant concentration and measurements done between 1 and 4 h after p … The choice of K2- or K3-EDTA as the preferred anticoagulant for blood count remains controversial.

Is there a difference between EDTA salt and anticoagulant after phlebotomy?

In optimal conditions (appropriate anticoagulant concentration and measurements done between 1 and 4 h after phlebotomy), no marked differences are seen between either EDTA salt. Important discrepancies appear, however, in less optimal conditions, as often happens in day to day practice.

How does anticoagulant concentration affect PCV?

Increasing anticoagulant concentration above 1.5 g/L led to decreased PCV for both K2 and K3EDTA specimens, but the effect was stronger in K3EDTA specimens.

How does anticoagulant concentration affect packed cell volume?

The packed cell volume, when measured on centrifuged blood, decreases with increasing anticoagulant concentrations and this is most pronounced with the K3 salt. This phenomenon has been reported by different authors and is ascribed to shrinking of erythrocytes in an hypertonic medium.