What is IVIS imaging?

What is IVIS imaging?

The IVIS Spectrum is the newest imaging system available from Caliper Life Sciences. Like previous IVIS systems, it uses Xenogen’s novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

What is in vivo imaging?

In vivo imaging is the non-invasive visualization of living organisms for research and/or diagnostic purposes. This method can be divided in two key areas: anatomical/morphological imaging and molecular imaging. Molecular imaging very often uses biomarkers labeled with bioluminescence or fluorescence.

How does IVIS work?

IVIS acquires a photographic image of the animal under white light and a quantitative bioluminescent or fluorescent signal, which is overlaid on the image. The bioluminescent signal is expressed in photons per second and displayed as an intensity map.

How much is an IVIS machine?

Estimated Cost: $620,000.00 – $650,000.00 (GST included) Delivery Date: Above-mentioned You are hereby notified that the government intends to negotiate with one firm only as identified above.

What is the difference between in vitro and in vivo?

In vitro and in vivo are two terms that you may encounter occasionally, particularly when reading about scientific studies. In vivo refers to when research or work is done with or within an entire, living organism. In vitro is used to describe work that’s performed outside of a living organism.

What is Vivo Microscopy?

In Vivo Microscopy (IVM) is an exciting field where microscopic images are obtained in vivo, in real-time, during clinical procedures. IVM imaging technologies use light and rapidly produce 2D or 3D (tomographic) microscopic images.

Where is luciferin found?

Bioluminescent dinoflagellates produce light using a luciferin-luciferase reaction. The luciferase found in dinoflagellates is related to the green chemical chlorophyll found in plants….Vocabulary.

Term Part of Speech Definition
milky sea noun phenomenon of bioluminescent bacteria on the surface of the ocean.

How do you make luciferin stock?

1. Prepare a 200X luciferin stock solution (30 mg/ml) in sterile water. Mix gently by inversion until luciferin is completely dissolved. Use immediately, or aliquot and freeze at -20°C for future use.

How do you photograph luciferase?


  1. Firefly luciferase requires D-luciferin to be injected into the subject prior to imaging.
  2. Renilla luciferase (from the Sea pansy) requires its substrate, coelenterazine, to be injected as well.

Why in vivo is better than in vitro?

In vitro studies use cells derived from animals or cell lines which have an infinite lifespan. The use of animals in in vivo studies addresses many of the shortcomings of in vitro studies. Scientists can better evaluate the safety, toxicity and efficacy of a drug candidate in a complex model.

What is Vivo X60?

Vivo X60 is a line of Android-based smartphones developed and manufactured by Vivo, it featured the Zeiss co-engineered imaging system.

Why is luciferin bad?

Luciferin Toxicity The small size of luciferin also makes it a poor antigen and immune responses to luciferin are unlikely. Luciferin is able to pass the blood brain barrier, the blood placenta barrier and the blood testis barrier, toxicity appears low.

What is the IVIS Lumina II?

The IVIS Lumina II from Caliper Life Sciences provides an expandable, sensitive imaging system that is easy to use for both fluorescent and bioluminescent imaging in vivo. The system includes a highly sensitive CCD camera, light-tight imaging chamber and complete automation and analysis capabilities.

What is Ivis Lumina™ X5?

The IVIS ® Lumina ™ X5 high-throughput 2D optical imaging system combines high-sensitivity bioluminescence and fluorescence with high-resolution x-ray into a compact system that fits on your benchtop.

What is the field of view of the IVIS Lumina S5?

The IVIS Lumina S5 integrates a 1 inch CCD camera into our benchtop instrument providing a high throughput 20 x 20 cm Field of View (FOV) sufficient for imaging 5 animals at a time for bioluminescence and fluorescence in vivo imaging.

What types of reporters can the IVIS Lumina III be used for?

The IVIS Lumina III is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped with up to 26 filter sets that can be used to image reporters that emit from green to near-infrared. Superior spectral unmixing can be achieved by Lumina III’s optional high resolution short cut off filters.